Western blot analysis of rat liver showing detection of ~47.2 kDa PTEN protein using Anti-PTEN Antibody (A305205) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: rat Liver. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~47.2 kDa. Other Band(s): 67 kDa.
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