Immunocytochemistry/Immunofluorescence analysis of rat dissociated hippocampal neurons, fixed in cold 4% paraformaldehyde / 0.2% glutaraldehyde in 0.1M sodium phosphate buffer, using Anti-PSD95 Antibody [6G6] (A304703), at 1:1,000 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at room temperature. Magnification: 10X.
Immunocytochemistry/Immunofluorescence analysis of human HaCaT cells, fixed in cold 100% methanol for 10 minutes at -20°C, using Anti-PSD95 Antibody [6G6] (A304703), at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Junction staining.
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