Anti-PRPF31 Antibody (A84273)

PRPF31 expression in A431 (A), HeLa (B) and HEK293 (C) nuclear cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-PRPF31 Antibody (A84273) at 1µg/ml and detected by chemiluminescence.

PRPF31 expression in NIH3T3 nuclear cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-PRPF31 Antibody (A84273) at 1µg/ml and detected by chemiluminescence.

PRPF31 expression in HEK293 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-PRPF31 Antibody (A84273) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Weak cytoplasmic and strong nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

PRPF31 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-PRPF31 Antibody (A84273) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Some cytoplasmic and strong nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.