Western blot analysis of extracts of various cell lines, using Anti-PRMT5 Antibody (A13485) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit.
Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-PRMT5 Antibody (A13485) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-PRMT5 Antibody (A13485) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-PRMT5 Antibody (A13485) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of H9C2 cells using Anti-PRMT5 Antibody (A13485) at dilution of 100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of L929 cells using Anti-PRMT5 Antibody (A13485) at dilution of 100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of U-2 OS cells using Anti-PRMT5 Antibody (A13485) at dilution of 100 (40x lens). DAPI was used to stain the cell nuclei (blue).
