Rabbit polyclonal antibody to PPGB (32k,cleaved Arg326).
Specificity
This antibody detects endogenous levels of fragment of activated PPGB resulting from cleavage adjacent to Arg326.
Applications
WB, ELISA
Dilutions
IHC: 1:50-1:100, ELISA: 1:20000
Reactivity
Human, Mouse
Immunogen
Synthetic peptide derived from human PPGB (amino acids 277-326).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
33kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Carboxypeptidase C, Carboxypeptidase L, CTSA, Lysosomal protective protein, PPGB, Protective protein cathepsin A, Protective protein for beta-galactosidase
Western Blot - Anti-PPGB (32k,cleaved Arg326) Antibody (A94233)
Western blot analysis of lysates from 293 and Jurkat cells, treated with etoposide 25uM 1h using Anti-PPGB (32k,cleaved Arg326) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
