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Anti-POLRMT Antibody (A88198)

Western Blot - Anti-POLRMT Antibody (A88198) - Antibodies.com Immunofluorescence - Anti-POLRMT Antibody (A88198) - Antibodies.com Immunofluorescence - Anti-POLRMT Antibody (A88198) - Antibodies.com Western Blot - Anti-POLRMT Antibody (A88198) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to POLRMT for WB, ICC/IF and IP.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-POLRMT Antibody
Description
Rabbit polyclonal antibody to POLRMT.
Applications
WB, ICC/IF, IP
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:50-1:100
Reactivity
Human, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 42-230 of human POLRMT (NP_005026.3).
Sequence
SSASPQEQDQDRRKDWGHVELLEVLQARVRQLQAESVSEVVVNRVDVARLPECGSGDGSLQPPRKVQMGAKDATPVPCGRWAKILEKDKRTQQMRMQRLKAKLQMPFQSGEFKALTRRLQVEPRLLSKQMAGCLEDCTRQAPESPWEEQLARLLQEAPGKLSLDVEQAPSGQHSQAQLSGQQQRLLAFF
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
139 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
DNA-directed RNA polymerase, mitochondrial, MtRPOL
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Western Blot - Anti-POLRMT Antibody (A88198) - Antibodies.comEnlarge Image

Western Blot - Anti-POLRMT Antibody (A88198)

Western blot analysis of extracts of various cell lines, using Anti-POLRMT Antibody (A88198) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunofluorescence - Anti-POLRMT Antibody (A88198) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-POLRMT Antibody (A88198)

Immunofluorescence analysis of C6 cells using Anti-POLRMT Antibody (A88198) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-POLRMT Antibody (A88198) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-POLRMT Antibody (A88198)

Immunofluorescence analysis of U-2 OS cells using Anti-POLRMT Antibody (A88198) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Western Blot - Anti-POLRMT Antibody (A88198) - Antibodies.comEnlarge Image

Western Blot - Anti-POLRMT Antibody (A88198)

Immunoprecipitation analysis of 200µg extracts of HepG2 cells using 3µg of Anti-POLRMT Antibody (A88198). This Western blot was performed on the immunoprecipitate using Anti-POLRMT Antibody (A88198) at a dilution of 1:1000.
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