Recombinant humanized monoclonal antibody to CD79b.
Applications
ELISA, FACS, Functional Assay, In Vivo
Reactivity
Human, Mouse, Cynomolgus Macaque
Host
Humanized
Clonality
Monoclonal
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A affinity chromatography.
Concentration
Reconstitution dependent.
Molecular Weight
This antibody has a predicted MW of 145.26 kDa.
Purity
> 95% (by SDS-PAGE and SEC-HPLC).
Product Form
Lyophilized
Reconstitution
Reconstitute with 100µl of sterile double-distilled water to bring antibody to 1mg/ml concentration. Gently shake to solubilize completely. Do not vortex!
Formulation
Lyophilized from 25mM Histadine, pH 6.2, with 8% Sucrose and 0.01% Tween80.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonyms
B-cell antigen receptor complex-associated protein beta chain, B-cell-specific glycoprotein B29, B29, Ig-beta, IGB, Immunoglobulin-associated B29 protein
SDS-PAGE analysis of Polatuzumab Biosimilar - Anti-CD79b Antibody - Low endotoxin, Azide free (A323672) under reducing (R) conditions confirms that the purity of this antibody is greater than 95%.
SEC-HPLC analysis of Polatuzumab Biosimilar - Anti-CD79b Antibody - Low endotoxin, Azide free (A323672) confirms that the purity of this antibody is 98.12%.
Immobilized recombinant human CD79b protein (His tag) at 16 µg/ml is bound by Polatuzumab Biosimilar - Anti-CD79b Antibody - Low endotoxin, Azide free (A323672). EC50 = 0.003729 µg/ml.
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