Recombinant chimeric rabbit monoclonal antibody to CD79b.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA 1:5,000-1:10,000, Flow Cytometry: 1:100
Reactivity
Human
Host
Chimeric Rabbit
Clonality
Monoclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Synonyms
B-cell antigen receptor complex-associated protein beta chain, B-cell-specific glycoprotein B29, B29, Ig-beta, IGB, Immunoglobulin-associated B29 protein
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
CD79b expresion in Expi293 cells compared to irrelevant protein as analyzed by flow cytometry. Staining was performed with 15µg/ml of Polatuzumab Biosimilar - Anti-CD79b Antibody to obtain the blue histogram or the red histogram for the irrelevant protein.
Immobilised Recombinant Human CD79b Protein (Fc Tag) (A317868) at 2 µg/ml (100 µl/well) can bind Polatuzumab Biosimilar - Anti-CD79b Antibody in a linear range of 0.13–80 ng/ml.
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