Anti-PKR (phospho Thr446) Antibody [ARC0293] (A306474)

Name: Anti-PKR (phospho Thr446) Antibody [ARC0293]
Brand: Antibodies.com
SKU: A306474
Price: 560.0 USD
Availability: InStock

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$560

Rabbit monoclonal [ARC0293] antibody to PKR (phospho Thr446) for WB.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-PKR (phospho Thr446) Antibody [ARC0293]

Description

Rabbit monoclonal [ARC0293] antibody to PKR (phospho Thr446).

Applications

Dilutions

WB: 1:500-1:2,000

Reactivity

Human

Immunogen

A synthetic phosphorylated peptide around T446 of human PKR/EIF2AK2E2AK2 (P19525).

Sequence

KRTRS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0293

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

74 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

EC:2.7.10.2, eIF-2A protein kinase 2, EIF2AK2, Eukaryotic translation initiation factor 2-alpha kinase 2, Interferon-induced, double-stranded RNA-activated protein kinase, Interferon-inducible RNA-dependent protein kinase, P1/eIF-2A protein kinase, p68 kinase, PRKR, Protein kinase R, Protein kinase RNA-activated, Tyrosine-protein kinase EIF2AK2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-PKR (phospho Thr446) Antibody [ARC0293] (A306474)

Western blot analysis of extracts of various cell lines, using Anti-PKR (phospho Thr446) Antibody [ARC0293] (A306474) at 1:1,000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Western Blot - Anti-PKR (phospho Thr446) Antibody [ARC0293] (A306474)

Western blot analysis of extracts of various cell lines, using Anti-PKR (phospho Thr446) Antibody [ARC0293] (A306474) at 1:1,000 dilution. Jurkat cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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