Anti-PKR (Phospho-Thr446) Antibody (A50216)

Name: Anti-PKR (Phospho-Thr446) Antibody
Brand: Antibodies.com
SKU: A50216
Price: 275.0 USD
Availability: InStock

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$275

Rabbit polyclonal antibody to PKR (Phospho-Thr446) for WB.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-PKR (Phospho-Thr446) Antibody

Description

Rabbit polyclonal antibody to PKR (Phospho-Thr446)

Specificity

The antibody detects endogenous level of PKR only when phosphorylated at threonine 446.

Applications

Dilutions

Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200

Reactivity

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 446 (K-R-T(p)-R-S) derived from Human PKR.

Host

Rabbit

Clonality

Polyclonal

Conjugate

Unconjugated

Purification

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/ml

Predicted MW

68kd

Formulation

Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Synonyms

EC:2.7.10.2, eIF-2A protein kinase 2, EIF2AK2, Eukaryotic translation initiation factor 2-alpha kinase 2, Interferon-induced, double-stranded RNA-activated protein kinase, Interferon-inducible RNA-dependent protein kinase, P1/eIF-2A protein kinase, p68 kinase, PRKR, Protein kinase R, Protein kinase RNA-activated, Tyrosine-protein kinase EIF2AK2

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Anti-PKR (Phospho-Thr446) Antibody (A50216)

Western blot analysis of extracts from K562 cells using PKR (Phospho-Thr446) Antibody #11280 (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).

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Immunohistochemistry - Anti-PKR (Phospho-Thr446) Antibody (A50216)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PKR (Phospho-Thr446) Antibody #11280 (left) or the same antibody preincubated with blocking peptide (right).