Anti-PKR (phospho Thr446) Antibody (A306475)

Name: Anti-PKR (phospho Thr446) Antibody
Brand: Antibodies.com
SKU: A306475
Price: 450.0 USD
Availability: InStock

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$450

Rabbit polyclonal antibody to PKR (phospho Thr446) for WB.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-PKR (phospho Thr446) Antibody

Description

Rabbit polyclonal antibody to PKR (phospho Thr446).

Applications

Dilutions

WB: 1:500-1:1,000

Reactivity

Human, Mouse

Immunogen

A synthetic phosphorylated peptide around T446 of human PKR/EIF2AK2 (NP_002750.1).

Sequence

KRTRS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

74 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

EC:2.7.10.2, eIF-2A protein kinase 2, EIF2AK2, Eukaryotic translation initiation factor 2-alpha kinase 2, Interferon-induced, double-stranded RNA-activated protein kinase, Interferon-inducible RNA-dependent protein kinase, P1/eIF-2A protein kinase, p68 kinase, PRKR, Protein kinase R, Protein kinase RNA-activated, Tyrosine-protein kinase EIF2AK2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (1)

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Western Blot - Anti-PKR (phospho Thr446) Antibody (A306475)

Western blot analysis of extracts of HeLa cells, using Anti-PKR (phospho Thr446) Antibody (A306475) at 1:1,000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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