Anti-PKR Antibody (A11716)

Name: Anti-PKR Antibody
Brand: Antibodies.com
SKU: A11716
Price: 345.0 USD
Availability: InStock

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$345

Rabbit polyclonal antibody to PKR for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-PKR Antibody

Description

Rabbit polyclonal antibody to PKR.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:1,000-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human PKR/EIF2AK2 (NP_002750.1).

Sequence

MAGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFTFQVIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKAVSPLLLTTTNSSEGLSMGNYIGLINRIAQKKRLTVNYEQCASGVHGPEGFHYKCKMGQKEYSIGTGSTKQEAKQLAAKLAYLQILSEETSVKSDYLSSGSFATTCESQSNSLVTSTLASESSSEGDFSADTSEINSNSDSLNSSSLLMNGLRNNQRKAKRSLAPRFDLPDMKETKYTVDKRFGMDFKEI

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

65 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

EC:2.7.10.2, eIF-2A protein kinase 2, EIF2AK2, Eukaryotic translation initiation factor 2-alpha kinase 2, Interferon-induced, double-stranded RNA-activated protein kinase, Interferon-inducible RNA-dependent protein kinase, P1/eIF-2A protein kinase, p68 kinase, PRKR, Protein kinase R, Protein kinase RNA-activated, Tyrosine-protein kinase EIF2AK2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-PKR Antibody (A11716)

Western blot analysis of extracts of MCF-7 cells, using Anti-PKR Antibody (A11716). The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST.

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Western Blot - Anti-PKR Antibody (A11716)

Western blot analysis of extracts of various cell lines, using Anti-PKR Antibody (A11716) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunohistochemistry - Anti-PKR Antibody (A11716)

Immunohistochemistry analysis of paraffin-embedded human liver using Anti-PKR Antibody (A11716) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.