Rabbit polyclonal antibody to PHB (Phospho-Thr258)
Specificity
The antibody detects endogenous level of PHB only when phosphorylated at threonine 258.
Applications
WB
Dilutions
Western blotting:1:500~1:1000
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of threonine 258 (N-I-T(p)-Y-L) derived from Human PHB.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.