Anti-PFKM Antibody (A14790)

Name: Anti-PFKM Antibody
Brand: Antibodies.com
SKU: A14790
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to PFKM for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-PFKM Antibody

Description

Rabbit polyclonal antibody to PFKM.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 501-780 of human PFKM (NP_001160160.1).

Sequence

AYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

85 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

6-phosphofructokinase type A, ATP-dependent 6-phosphofructokinase, muscle type, ATP-PFK, PFK-A, PFKX, Phosphofructo-1-kinase isozyme A, Phosphohexokinase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-PFKM Antibody (A14790)

Western blot analysis of extracts of various cell lines, using Anti-PFKM Antibody (A14790) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunofluorescence - Anti-PFKM Antibody (A14790)

Immunofluorescence analysis of C6 cells using Anti-PFKM Antibody (A14790) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-PFKM Antibody (A14790)

Immunofluorescence analysis of L929 cells using Anti-PFKM Antibody (A14790) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-PFKM Antibody (A14790)

Immunofluorescence analysis of NIH-3T3 cells using Anti-PFKM Antibody (A14790) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).