Anti-PF4 Antibody (A92672)

Name: Anti-PF4 Antibody
Brand: Antibodies.com
SKU: A92672
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to PF4 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-PF4 Antibody

Description

Rabbit polyclonal antibody to PF4.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 32-101 of human CXCL4/PF4 (NP_002610.1).

Sequence

EAEEDGDLQCLCVKTTSQVRPRHITSLEVIKAGPHCPTAQLIATLKNGRKICLDLQAPLYKKIIKKLLES

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

10 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

C-X-C motif chemokine 4, CXCL4, Iroplact, Oncostatin-A, PF-4, Platelet factor 4, SCYB4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-PF4 Antibody (A92672)

Western blot analysis of extracts of various cell lines, using Anti-PF4 Antibody (A92672) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Immunohistochemistry - Anti-PF4 Antibody (A92672)

Immunohistochemistry analysis of paraffin-embedded rat bone marrow using Anti-PF4 Antibody (A92672) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-PF4 Antibody (A92672)

Immunofluorescence analysis of rat bone marrow using Anti-PF4 Antibody (A92672) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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