Western Blot - Anti-PERK (phospho Thr982) Antibody (A88555)
Western blot analysis of extracts of 293T cells, using Anti-PERK (phospho Thr982) Antibody (A88555) at 1:1,000 dilution. 293T cells were treated by IGF-1 (50 ng/ml) at 37°C for 5 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 90s.
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