Anti-Peripherin Antibody (A85435)

These are Coomassie Brilliant Blue stained whole protein extracts of adult rat cortex (Lane 1), brain stem (Lane 2), cerebellum (Lane 3), and spinal cord (Lane 4) separated on 8% SDS-PAGE. The major neurofilament subunits are indicated by “NF-L”, “NF-M” and ”NF-H”, spectrin/fodrin by “S/F” and Microtubule associated protein 2 by “MAP2”. Right Panel is a western blot of similar preparations in the reverse orientation, where Lane 1 is spinal cord, Lane 2 is brain stem, Lane three is cerebellum and Lane 4 is cortex. This blot is from a 6% SDS-PAGE and processed for immunoblotting with Anti-Peripherin Antibody, at a dilution of 1:10,000). A clear band running at 57kDa apparent size is seen in the spinal cord and brain stem lanes, but not in the other regions, in which peripherin is a very minor component. The position of this band on the Coomassie stained preparation is a little higher on the 8% gel and is indicated by “Peri” in the left panel.

Immunohistochemistry analysis of a formalin fixed paraffin embedded rat brain stem section with Anti-Peripherin Antibody (A85435) at a dilution of 1:20,000 detected in DAB (brown) following the ABC method. Counterstained with Hematoxylin (blue). In the central nervous system, peripherin antibody labels axonal bundles which project into the peripheral nervous system.