Anti-Peripherin Antibody (A85430)

Immunohistochemistry analysis of a NBF fixed paraffin embedded human midbrain section with Anti-Peripherin Antibody (A85430) at a dilution of 1:2,000 detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Counterstained with Hematoxylin (blue). Anti-Peripherin Antibody (A85430) strongly labels the cytoplasm of neuronal peripheral projections. Note: this antibody performs well in testing with 4% PFA and standard NBF fixed mouse, rat and human tissue.

Mixed neuron/glia cultures from newborn rat brain stained with Anti-Peripherin Antibody (green) and Anti-NF-H Antibody (A85337 | red). A class of large neurons, like the one at the top right of this image, contain peripherin, while the majority of neurons and their processes contain NF-H and not peripherin. The blue channel reveals the localization of DNA.

Immunofluorescent analysis of the widely used rat pheochromocytoma PC12 cell line stained with Anti-Peripherin Antibody (A85430), at a dilution of 1:2,000, in green. The nuclear DNA is visualised in blue using Hoechst staining. Peripherin, one of the Class III family of intermediate filament subunit proteins, is a major component of the PC12 cell, forming a perinuclear cap with some filaments visible in the cytoplasm.

Western blot analysis of tissue and cell lysates using Anti-Peripherin Antibody (A85430), at a dilution of 1:10,000 in green. The lanes contain samples of: [Lane 1] Protein standards, [Lane 2] rat spinal cord, [Lane 3] mouse spinal cord, [Lane 4] pig spinal cord, [Lane 5] cow spinal cord, [Lane 6] SH-SY5Y cells and [Lane 7] PC12 cells. The major band at approximately 57 kDa corresponds to the major peripherin protein isoform, while other bands presumably represent protein products of alternate transcripts of the peripherin gene.