Recombinant Anti-Perforin Antibody [rPRF1/8058] (A315502)

Anti-Perforin Antibody [rPRF1/8058]

Recombinant mouse monoclonal [rPRF1/8058] antibody to Perforin.

Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. It is one of the main cytolytic proteins of cytolytic granules, and is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The expression of perforin is reportedly upregulated in activated CD8+ T-cells, natural killer cells and some CD4+ T-cells.

WB, IHC-P

HDLM-2 cells. Human spleen.

WB: 1-2 µg/ml, IHC: 1-2 µg/ml

Human

Recombinant human Perforin-1 protein fragment (around Amino Acids 355-555). The exact sequence is proprietary.

Mouse

Monoclonal

rPRF1/8058

kappa

Protein A/G chromatography.

200 µg/ml

75 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Cytolysin, Lymphocyte pore-forming protein, P1, Perforin-1, PFP, PRF1