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Pepinemab Biosimilar - Anti-Semaphorin 4D/CD100 Antibody - Low endotoxin, Azide free (A323659)

SDS-PAGE - Pepinemab Biosimilar - Anti-Semaphorin 4D/CD100 Antibody - Low endotoxin, Azide free (A323659) - Antibodies.com SEC-HPLC - Pepinemab Biosimilar - Anti-Semaphorin 4D/CD100 Antibody - Low endotoxin, Azide free (A323659) - Antibodies.com ELISA - Pepinemab Biosimilar - Anti-Semaphorin 4D/CD100 Antibody - Low endotoxin, Azide free (A323659) - Antibodies.com FACS - Pepinemab Biosimilar - Anti-Semaphorin 4D/CD100 Antibody - Low endotoxin, Azide free (A323659) - Antibodies.com Research in vivo - Pepinemab Biosimilar - Anti-Semaphorin 4D/CD100 Antibody - Low endotoxin, Azide free (A323659) - Antibodies.com
Recombinant Antibody
$305
Product Datasheet

Recombinant human monoclonal antibody to Semaphorin 4D/CD100 for ELISA, FACS, Functional Assay and In Vivo.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 12-18 business days.

  • Specifications

Name
Pepinemab Biosimilar - Anti-Semaphorin 4D/CD100 Antibody - Low endotoxin, Azide free
Description
Recombinant human monoclonal antibody to Semaphorin 4D/CD100.
Applications
ELISA, FACS, Functional Assay, In Vivo
Reactivity
Human, Mouse
Host
Human
Clonality
Monoclonal
Isotype
IgG4SP - Human IgG4 with S228P mutation
Light Chains
kappa
Conjugate

Unconjugated

Purification
Protein A affinity chromatography.
Concentration
Reconstitution dependent.
Molecular Weight
This antibody has a predicted MW of 145.88 kDa.
Purity
> 95% (by SDS-PAGE and SEC-HPLC).
Product Form
Lyophilized
Reconstitution
Reconstitute with 100µl of sterile double-distilled water to bring antibody to 1mg/ml concentration. Gently shake to solubilize completely. Do not vortex!
Formulation
Lyophilized from 25mM Histadine, pH 6.2, with 8% Sucrose and 0.01% Tween80.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Suitable Secondaries
See all Anti-Human IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (5)

SDS-PAGE - Pepinemab Biosimilar - Anti-Semaphorin 4D/CD100 Antibody - Low endotoxin, Azide free (A323659) - Antibodies.comEnlarge Image

SDS-PAGE - Pepinemab Biosimilar - Anti-Semaphorin 4D/CD100 Antibody - Low endotoxin, Azide free (A323659)

SDS-PAGE analysis of Pepinemab Biosimilar - Anti-Semaphorin 4D/CD100 Antibody - Low endotoxin, Azide free (A323659) under reducing (R) conditions confirms that the purity of this antibody is greater than 95%.
SEC-HPLC - Pepinemab Biosimilar - Anti-Semaphorin 4D/CD100 Antibody - Low endotoxin, Azide free (A323659) - Antibodies.comEnlarge Image

Pepinemab Biosimilar - Anti-Semaphorin 4D/CD100 Antibody - Low endotoxin, Azide free (A323659)

SEC-HPLC analysis of Pepinemab Biosimilar - Anti-Semaphorin 4D/CD100 Antibody - Low endotoxin, Azide free (A323659) confirms that the purity of this antibody is 100%.
ELISA - Pepinemab Biosimilar - Anti-Semaphorin 4D/CD100 Antibody - Low endotoxin, Azide free (A323659) - Antibodies.comEnlarge Image

Pepinemab Biosimilar - Anti-Semaphorin 4D/CD100 Antibody - Low endotoxin, Azide free (A323659)

Immobilized recombinant human SEMA4D/CD100 protein (His tag) at 2 µg/ml is bound by Pepinemab Biosimilar - Anti-Semaphorin 4D/CD100 Antibody - Low endotoxin, Azide free (A323659). EC50 = 0.0081 µg/ml.
FACS - Pepinemab Biosimilar - Anti-Semaphorin 4D/CD100 Antibody - Low endotoxin, Azide free (A323659) - Antibodies.comEnlarge Image

Pepinemab Biosimilar - Anti-Semaphorin 4D/CD100 Antibody - Low endotoxin, Azide free (A323659)

Human C100 HEK293 cells were stained with Pepinemab Biosimilar - Anti-Semaphorin 4D/CD100 Antibody - Low endotoxin, Azide free (A323659) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50 = 0.3227 nM.
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