Anti-PEA15 Antibody [4D117] (A270552)

Immunofluorescent analysis of rat brain section stained with Anti-PEA15 Antibody [4D117] (A270552), at a dilution of 1:1,000, in red, and co-stained with Anti-GFAP Antibody (A85419), at a dilution of 1:5,000, in green. Nuclear DNA is visualised in blue using Hoechst staining. Following transcardial perfusion with 4% paraformaldehyde, the brain was post-fixed for 24 hours, cut to 45 µm, and free-floating sections were stained using the above antibodies. Anti-PEA15 Antibody [4D117] (A270552) labels the cytoplasm of certain presumably neuronal cells which are not labelled by the astrocyte specific Anti-GFAP Antibody (A85419).

Western blot analysis of different tissue lysates using Anti-PEA15 Antibody [4D117] (A270552), at a dilution of 1:1,000, in green. The lanes contain: [Lane 1] protein standard (red(, [Lane 2] rat whole brain, [Lane 3] rat cerebellum, [Lane 4] mouse whole brain, [Lane 5] cow cortex, and [Lane 6] cow cerebellum. The strong band at about 15 kDa corresponds to the PEA15 protein.

Immunohistochemistry analysis of a 4% PFA fixed paraffin embedded rat testes section with Anti-PEA15 Antibody [4D117] (A270552) at a dilution of 1:1,000 detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Counterstained with Hematoxylin (blue). Note: this antibody performs well in testing with 4% PFA but does not stain long term NBF fixed tissue effectively.