Anti-PDK1 Antibody [ARC52376] (A308815)

Name: Anti-PDK1 Antibody [ARC52376]
Brand: Antibodies.com
SKU: A308815
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC52376] antibody to PDK1 for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-PDK1 Antibody [ARC52376]

Description

Rabbit monoclonal [ARC52376] antibody to PDK1.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 337-436 of human PDK1/PDHK1 (NP_002601.1).

Sequence

STAPRPRVETSRAVPLAGFGYGLPISRLYAQYFQGDLKLYSLEGYGTDAVIYIKALSTDSIERLPVYNKAAWKHYNTNHEADDWCVPSREPKDMTTFRSA

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC52376

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

49 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

PDH kinase 1, PDHK1, Pyruvate dehydrogenase kinase isoform 1, [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-PDK1 Antibody [ARC52376] (A308815)

Western blot analysis of extracts of various cell lines, using Anti-PDK1 Antibody [ARC52376] (A308815) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Immunohistochemistry - Anti-PDK1 Antibody [ARC52376] (A308815)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-PDK1 Antibody [ARC52376] (A308815) at a dilution of 1:1,000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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