Anti-PDGF B Antibody [MOR-8457] - Low endotoxin, Azide free
Description
Recombinant human monoclonal [MOR-8457] antibody to PDGF B.
Applications
ELISA, FACS, Functional Assay, In Vivo
Reactivity
Human, Mouse, Cynomolgus Macaque
Host
Human
Clonality
Monoclonal
Clone ID
MOR-8457
Isotype
IgG1
Light Chains
lambda
Conjugate
Unconjugated
Purification
Protein A affinity chromatography.
Concentration
Reconstitution dependent.
Molecular Weight
This antibody has a predicted MW of 145 kDa.
Purity
> 90% (by SDS-PAGE and SEC-HPLC).
Product Form
Lyophilized
Reconstitution
Reconstitute with 100µl of sterile double-distilled water to bring antibody to 1mg/ml concentration. Gently shake to solubilize completely. Do not vortex!
Formulation
Lyophilized from 25mM Histadine, pH 6.2, with 8% Sucrose and 0.01% Tween80.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
SDS-PAGE analysis of Anti-PDGF B Antibody [MOR-8457] - Low endotoxin, Azide free (A324183) under reducing (R) conditions confirms that the purity of this antibody is greater than 90%.
Anti-PDGF B Antibody [MOR-8457] - Low endotoxin, Azide free (A324183)
SEC-HPLC analysis of Anti-PDGF B Antibody [MOR-8457] - Low endotoxin, Azide free (A324183) confirms that the purity of this antibody is greater than 95%.
Anti-PDGF B Antibody [MOR-8457] - Low endotoxin, Azide free (A324183)
Immobilized recombinant human PDGFB protein (Fc tag) at 2 µg/mL is bound by Anti-PDGF B Antibody [MOR-8457] - Low endotoxin, Azide free (A324183). EC50 was approximately 0.07279 µg/ml.
Anti-PDGF B Antibody [MOR-8457] - Low endotoxin, Azide free (A324183)
Immobilized recombinant mouse PDGF B protein (His tag) at 2 µg/mL is bound by Anti-PDGF B Antibody [MOR-8457] - Low endotoxin, Azide free (A324183). EC50 was approximately 0.008642 µg/ml.
Anti-PDGF B Antibody [MOR-8457] - Low endotoxin, Azide free (A324183)
Immobilized recombinant cynomolgus/rhesus macaque PDGF B protein (Fc tag) at 2 µg/mL is bound by Anti-PDGF B Antibody [MOR-8457] - Low endotoxin, Azide free (A324183). EC50 was approximately 0.06539 µg/ml.
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