Western blot analysis of mouse brain showing detection of 27.3 kDa PDGFB protein using Anti-PDGF B Antibody (A304924) at 1:1,000 for 2 hours at room temperature with shaking. Load: 15 µg. Block: 5% Skim Milk powder in TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: 27.3 kDa. Other Band(s): 25 kDa.
Immunohistochemistry - Anti-PDGF B Antibody (A304924)
Immunohistochemistry analysis of human heart, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-PDGF B Antibody (A304924) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
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