Mouse monoclonal [EH12.2H7] antibody to PD1 (PE-Cyanine 7).
Specificity
This antibody recognizes an extracellular epitope of CD279, a 55 kDa type I transmembrane protein expressed above all during T cell development, on activated T cells, activated B cells, and activated monocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human, Non-Human Primates
Immunogen
Unfortunately, this information is considered to be commercially sensitive.
Host
Mouse
Clonality
Monoclonal
Clone ID
EH12.2H7
Isotype
IgG1
Light Chains
kappa
Conjugate
PE-Cyanine 7
Excitation: 565nm, Emission: 778nm
Purification
This antibody is conjugated with tandem dye PE-Cyanine 7 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
55 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
CD279, PDCD1, Programmed cell death protein 1, Protein PD-1
Separation of human PHA stimulated peripheral blood mononuclear cells stained with Anti-CD279 Antibody [EH12.2H7] (PE-Cyanine 7) (4µl reagent per milion cells in 100µl of cell suspension, red-filled) from human PHA stimulated peripheral blood mononuclear cells stained with Mouse IgG1 [MOPC-21] (PE-Cyanine 7) (Isotype Control) (concentration in sample is 1.67 µg/ml, same as Anti-CD279 Antibody [EH12.2H7] (PE-Cyanine 7) concentration, black-dashed) in flow cytometry analysis.
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![Flow Cytometry - Anti-CD279 Antibody [EH12.2H7] (PE-Cyanine 7) (A122004) - Antibodies.com](https://cdn.antibodies.com/image/catalog/122/A122004_2.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-CD279 Antibody [EH12.2H7] (PE-Cyanine 7) (A122003) - Antibodies.com](https://cdn.antibodies.com/image/catalog/122/A122004_1.jpg?profile=product_image)
![Flow Cytometry - Anti-CD279 Antibody [EH12.2H7] (PE-Cyanine 7) (A122004) - Antibodies.com](https://cdn.antibodies.com/image/catalog/122/A122004_2.jpg?profile=product_image)