Anti-PD-L2 Antibody [TY25] (FITC) (A281863)

Anti-PD-L2 Antibody [TY25] (FITC)

Rat monoclonal [TY25] antibody to PD-L2 (FITC).

This antibody recognises mouse CD273, also known as B7-dendritic cell (B7-DC), or programmed death ligand-2 (PD-L2). CD273 is a 247 amino acid, ~42 kDa member of the B7 family. The expression of CD273 is restricted to a subpopulation of dendritic cells (DCs), and DCs and macrophages that have been stimulated with interferon gamma, GM-CSF or IL-4.CD273 has been identified as a ligand for PD-1(CD279), along with another B7 family member B7-H1 (CD274). CD273 and B7-H1 co-stimulate or inhibit T cell proliferation and cytokine production. Rat anti Mouse CD273 antibody, clone TY25 is reported to block the binding of CD273 (B7-DC) to it's receptor.

Flow Cytometry: Neat - 1:5, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.

Mouse

B7-DC transfected RAW264.7 cells.

Rat

Monoclonal

TY25

IgG2a

Protein G affinity chromatography of tissue culture supernatant.

100 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Rat anti Mouse CD273 antibody, clone TY25 recognises mouse CD273, also known as B7-dendritic cell (B7-DC), or programmed death ligand-2 (PD-L2). CD273 is a 247 amino acid, ~42 kDa member of the B7 family. The expression of CD273 is restricted to a subpopulation of dendritic cells (DCs), and DCs and macrophages that have been stimulated with interferon gamma, GM-CSF or IL-4.CD273 has been identified as a ligand for PD-1(CD279), along with another B7 family member B7-H1 (CD274). CD273 and B7-H1 co-stimulate or inhibit T cell proliferation and cytokine production (Schmidt et al.2015).Rat anti Mouse CD273 antibody, clone TY25 is reported to block the binding of CD273 (B7-DC) to it's receptor (Matsumoto et al. 2004).

B7-DC, B7DC, Butyrophilin B7-DC, CD273, PD-1 ligand 2, PDCD1 ligand 2, PDCD1L2, PDCD1LG2, PDL2, Programmed cell death 1 ligand 2, Programmed death ligand 2

This product is for research use only. It is not intended for diagnostic or therapeutic use.