This antibody recognises mouse CD273, also known as B7-dendritic cell (B7-DC), or programmed death ligand-2 (PD-L2). CD273 is a 247 amino acid, ~42 kDa member of the B7 family. The expression of CD273 is restricted to a subpopulation of dendritic cells (DCs), and DCs and macrophages that have been stimulated with interferon gamma, GM-CSF or IL-4.CD273 has been identified as a ligand for PD-1(CD279), along with another B7 family member B7-H1 (CD274). CD273 and B7-H1 co-stimulate or inhibit T cell proliferation and cytokine production. Rat anti Mouse CD273 antibody, clone TY25 is reported to block the binding of CD273 (B7-DC) to it's receptor.
Applications
IP, Flow Cytometry, IHC-Fr
Dilutions
Flow Cytometry: 1:100 - 1:200, Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Reactivity
Mouse
Immunogen
B7-DC transfected RAW264.7 cells.
Host
Rat
Clonality
Monoclonal
Clone ID
TY25
Isotype
IgG2a
Conjugate
Unconjugated
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rat anti Mouse CD273 antibody, clone TY25 recognises mouse CD273, also known as B7-dendritic cell (B7-DC), or programmed death ligand-2 (PD-L2). CD273 is a 247 amino acid, ~42 kDa member of the B7 family. The expression of CD273 is restricted to a subpopulation of dendritic cells (DCs), and DCs and macrophages that have been stimulated with interferon gamma, GM-CSF or IL-4.CD273 has been identified as a ligand for PD-1(CD279), along with another B7 family member B7-H1 (CD274). CD273 and B7-H1 co-stimulate or inhibit T cell proliferation and cytokine production (Schmidt et al.2015).Rat anti Mouse CD273 antibody, clone TY25 is reported to block the binding of CD273 (B7-DC) to it's receptor (Matsumoto et al. 2004).