Mouse monoclonal [24F.10C12] antibody to PD-L2 (APC).
Specificity
This antibody recognizes an extracellular epitope of CD273, a 25 kDa type I transmembrane protein expressed by dendritic cells, activated monocytes and T cells, heart, first trimester placenta, lung and liver, as well as in Hodgkin´s lymphoma cells and primary mediastinal B cell lymphoma (PMBL).
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Human CD273.
Host
Mouse
Clonality
Monoclonal
Clone ID
24F.10C12
Isotype
IgG2a
Light Chains
kappa
Conjugate
APC
Excitation: 645nm, Emission: 660nm
Purification
This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
25 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Flow cytometric analysis of human stimulated monocytes (GM-CSF + IL-4) stained with Anti-CD273 Antibody [24F.10C12] (APC) (10µl reagent per milion cells in 100µl of cell suspension).
Separation of human dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from non-stimulated lymphocytes (black-dashed) in flow cytometry analysis of human stimulated monocytes (GM-CSF + IL-4) using Anti-CD273 Antibody [24F.10C12] (APC) (10µl reagent per 100µl of peripheral whole blood).
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![Flow Cytometry - Anti-CD273 Antibody [24F.10C12] (APC) (A121897) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121898_1.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-CD273 Antibody [24F.10C12] (APC) (A121898) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121898_2.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-CD273 Antibody [24F.10C12] (APC) (A121897) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121898_1.jpg?profile=product_image)
![Flow Cytometry - Anti-CD273 Antibody [24F.10C12] (APC) (A121898) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121898_2.jpg?profile=product_image)