Recombinant rabbit monoclonal [PCNA/8303R] antibody to PCNA.
Specificity
Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.
Applications
WB, IHC-P
Positive Control
Tonsil or reactive lymph node. SKBr3, HEK293 or U937 cells.
Dilutions
WB: 1-2 µg/ml, IHC: 1-2 µg/ml
Reactivity
Human
Immunogen
Recombinant full-length human PCNA protein.
Host
Rabbit
Clonality
Monoclonal
Clone ID
PCNA/8303R
Isotype
IgG
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
36 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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