This antibody recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.
Applications
WB, IHC-P
Dilutions
WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml
Reactivity
Human, Mouse, Rat, Yeast
Immunogen
Rat PCNA/protein A fusion protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
PC5
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
1 mg/ml
Molecular Weight
36 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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![Immunohistochemistry - Anti-PCNA Antibody [PC5] - BSA and Azide free (A252760) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252761_1.jpg?profile=product_top_thumb)
![Immunohistochemistry - Anti-PCNA Antibody [PC5] - BSA and Azide free (A252761) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252761_2.jpg?profile=product_top_thumb)
![Western Blot - Anti-PCNA Antibody [PC5] - BSA and Azide free (A252761) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252761_3.jpg?profile=product_top_thumb)
![Immunohistochemistry - Anti-PCNA Antibody [PC5] - BSA and Azide free (A252760) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252761_1.jpg?profile=product_image)
![Immunohistochemistry - Anti-PCNA Antibody [PC5] - BSA and Azide free (A252761) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252761_2.jpg?profile=product_image)
![Western Blot - Anti-PCNA Antibody [PC5] - BSA and Azide free (A252761) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252761_3.jpg?profile=product_image)
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