PCNA expression in HeLa cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-PCNA Antibody (A83561) at 0.01µg/ml and detected by chemiluminescence.
PCNA expression in NIH3T3 (A), Mouse Testes (B) and Rat Testes (C) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-PCNA Antibody (A83561) at 0.01µg/ml and detected by chemiluminescence.
PCNA expression in Pig Spleen lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-PCNA Antibody (A83561) at 0.03µg/ml and detected by chemiluminescence.
PCNA expression in Human Testis analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by heating in citrate buffer, pH 6. Staining was performed with Anti-PCNA Antibody (A83561) at 6µg/ml and revealed with horseradish peroxidase (HRP).
Negative control for PCNA expression in Human Testis analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.
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