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Anti-PCNA Antibody (A359)

Western Blot of PCNA in HeLa cells and Xenopus egg extract. Anti-PCNA Antibody was used at 1:1,000 dilution. Goat Anti-Rabbit IgG Antibody (HRP) was used as a secondary at 1:10,000 dilution. Lane 1: HeLa cell extract (20µg). Lane 2: Xenopus egg extract (20µg). Molecular mass of PCNA is 29 kDa. Western blot of PCNA in the whole cell extracts. Anti-PCNA Antibody was used at 1:1,000 dilution. Goat Anti-Rabbit IgG Antibody (HRP) was used as secondary at 1:10,000 dilution. Lane 1: Full-size recombinant human PCNA protein (5ng). Lane 2: HeLa (human) (20µg). Lane 3: MCF7 (human) (20µg). Lane 4: NIH3T3 (mouse) (20µg). Lane 5: CHO (hamster) (20µg). Lane 6: Xenopus laevis egg (frog) (20µg). Immunofluorescence staining of PCNA in GM0637 cells (human fibroblast ). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.5% Triton and added with Anti-PCNA Antibody at 1:100 dilution. Goat Anti-Rabbit IgG Antibody (Alexa 488) was used as secondary at 1:10,000 dilution. DNA was stained with Heachst. PCNA foci were detected in the nuclei.
$390

Rabbit polyclonal antibody to PCNA for WB, IP, ICC/IF, DB and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.

  • Specifications

Name
Anti-PCNA Antibody
Description
Rabbit polyclonal antibody to PCNA.
Applications
WB, IP, ICC/IF, DB, ELISA
Dilutions
WB: 1:1,000-1:2,000, IP: 1:100-1:500, ICC/IF: 1:100-1:1,000, DB: 1:500-1:2,000, ELISA: 1:500-1:2,000
Reactivity
Human, Mouse, Rat, Hamster, Chicken, Xenopus
Immunogen
Recombinant full length human PCNA protein.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol (filter sterilised, without Sodium Azide, and carrier free).
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Cyclin, Proliferating cell nuclear antigen
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot of PCNA in HeLa cells and Xenopus egg extract. Anti-PCNA Antibody was used at 1:1,000 dilution. Goat Anti-Rabbit IgG Antibody (HRP) was used as a secondary at 1:10,000 dilution. Lane 1: HeLa cell extract (20µg). Lane 2: Xenopus egg extract (20µg). Molecular mass of PCNA is 29 kDa.Enlarge Image

Western Blot - Anti-PCNA Antibody (A359)

Anti-PCNA Antibody
Western blot of PCNA in the whole cell extracts. Anti-PCNA Antibody was used at 1:1,000 dilution. Goat Anti-Rabbit IgG Antibody (HRP) was used as secondary at 1:10,000 dilution. Lane 1: Full-size recombinant human PCNA protein (5ng). Lane 2: HeLa (human) (20µg). Lane 3: MCF7 (human) (20µg). Lane 4: NIH3T3 (mouse) (20µg). Lane 5: CHO (hamster) (20µg). Lane 6: Xenopus laevis egg (frog) (20µg).Enlarge Image

Anti-PCNA Antibody (A359)

Anti-PCNA Antibody
Immunofluorescence staining of PCNA in GM0637 cells (human fibroblast ). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.5% Triton and added with Anti-PCNA Antibody at 1:100 dilution. Goat Anti-Rabbit IgG Antibody (Alexa 488) was used as secondary at 1:10,000 dilution. DNA was stained with Heachst. PCNA foci were detected in the nuclei.Enlarge Image

Immunofluorescence - Anti-PCNA Antibody (A359)

Anti-PCNA Antibody
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