Western blot analysis of human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~28.7 kDa PCNA protein using Anti-PCNA Antibody (A304847) at 1:1,000 for 1 hour. Lane 1: Molecular Weight Ladder (MW). Lane 2: human 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit HRP at 1:2000 for 1 hour room temperature with shaking. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: ~28.7 kDa.
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