Anti-PCNA Antibody (A283116)

Anti-PCNA Antibody

Rabbit polyclonal antibody to PCNA.

This antibody recognises human Proliferating Cell Nuclear Antigen (PCNA), also known as cyclin. PCNA is a 261 amino acid ~36 kDa nuclear protein vital for cellular DNA synthesis, playing a key role in the DNA damage response where it acts as a loading platform to recruit additional damage repair proteins to lesions following DNA damage and promote repair. PCNA is a useful marker for DNA synthesis and is highly conserved among most species. Rabbit anti human PCNA antibody is expected to have wide species cross reactivity. PCNA expression in human dermis is negatively correlated to age as demonstrated by immunohistochemistry on paraffin sections using rabbit anti human PCNA antibody.

WB, IHC-P, ELISA

ELISA: 1:2,000 - 1:8,000, IHC-P: 2.5 µg/ml, WB: 1:500 - 1:2,000

Human, Chicken, Fish, Rat, Amphibia, Bovine, Monkey, Mouse, Dog

Synthetic peptide corresponding to the internal region of human PCNA protein.

Rabbit

Polyclonal

IgG

Liquid

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rabbit anti Human PCNA antibody recognizes Human Proliferating Cell Nuclear Antigen (PCNA), also known as cyclin. PCNA is a 261 amino acid ~36 kDa nuclear protein vital for cellular DNA synthesis, playing a key role in the DNA damage response where it acts as a loading platform to recruit additional damage repair proteins to lesions following DNA damage and promote repair (Burkovics et al. 2009).PCNA is a useful marker for DNA synthesis and is highly conserved among most species. Rabbit anti human PCNA antibody is expected to have wide species cross reactivity. PCNA expression in human dermis is negatively correlated to age as demonstrated by immunohistochemistry on paraffin sections using rabbit anti human PCNA antibody (Gunin et al. 2014).

Cyclin, Proliferating cell nuclear antigen