Anti-PCK2 Antibody (A306806)

Name: Anti-PCK2 Antibody
Brand: Antibodies.com
SKU: A306806
Price: 485.0 USD
Availability: InStock

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$485

Rabbit polyclonal antibody to PCK2 for WB, IHC, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Knockout Validated Antibody

Specifications

Name

Anti-PCK2 Antibody

Description

Rabbit polyclonal antibody to PCK2.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200, IHC: 1:50-1:200, IP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PEPCK/PCK2 (NP_004554.2).

Sequence

MAALYRPGLRLNWHGLSPLGWPSCRSIQTLRVLSGDLGQLPTGIRDFVEHSARLCQPEGIHICDGTEAENTATLTLLEQQGLIRKLPKYNNCWLARTDPKDVARVESKTVIVTPSQRDTVQLPPGGARGQLGNWMSPADFQRAVDERFPGCMQGRTMYVLPFSMGPVGSPLSRIGVQLTDSAYVVASMRIMTRLGTPVLQALGDGDFVKCLHSVGQPLTGQGEPVSQWPCNPEKTLIGHVPDQREIISFGSGYGGNSLLGKKCFALRIASRLARDEGWLA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

71 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

mtPCK2, PEPCK-M, PEPCK2, Phosphoenolpyruvate carboxykinase 2, mitochondrial, Phosphoenolpyruvate carboxykinase [GTP], mitochondrial

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (7)

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Western Blot - Anti-PCK2 Antibody (A306806)

Western blot analysis of various lysates, using Anti-PCK2 Antibody (A306806) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Western Blot - Anti-PCK2 Antibody (A306806)

Western blot analysis of extracts from wild type(WT) and PEPCK/PCK2 Rabbit polyclonal antibody knockout (KO) 293T cells, using Anti-PCK2 Antibody (A306806) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Immunohistochemistry - Anti-PCK2 Antibody (A306806)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-PCK2 Antibody (A306806) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-PCK2 Antibody (A306806)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-PCK2 Antibody (A306806) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.