Anti-PAX6 Antibody (A83369)

PAX6 expression in U251 (A), K562 nuclear (B), and HeLa nuclear (C) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-PAX6 Antibody (A83369) at 0.3µg/ml (A), 0.03µg/ml (B), or 1µg/ml (C) and detected by chemiluminescence.

PAX6 expression in RAW2647 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-PAX6 Antibody (A83369) at 0.5µg/ml and detected by chemiluminescence.

PAX6 expression in U251 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-PAX6 Antibody (A83369) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.