Anti-PARP1 Antibody (A87972)

Name: Anti-PARP1 Antibody
Brand: Antibodies.com
SKU: A87972
Price: 485.0 USD
Availability: InStock

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$485

Rabbit polyclonal antibody to PARP1 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-PARP1 Antibody

Description

Rabbit polyclonal antibody to PARP1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 662-1014 of PARP1 (NP_001609.2).

Sequence

KSKLPKPVQDLIKMIFDVESMKKAMVEYEIDLQKMPLGKLSKRQIQAAYSILSEVQQAVSQGSSDSQILDLSNRFYTLIPHDFGMKKPPLLNNADSVQAKVEMLDNLLDIEVAYSLLRGGSDDSSKDPIDVNYEKLKTDIKVVDRDSEEAEIIRKYVKNTHATTHNAYDLEVIDIFKIEREGECQRYKPFKQLHNRRLLWHGSRTTNFAGILSQGLRIAPPEAPVTGYMFGKGIYFADMVSKSANYCHTSQGDPIGLILLGEVALGNMYELKHASHISKLPKGKHSVKGLGKTTPDPSANISLDGVDVPLGTGISSGVNDTSLLYNEYIVYDIAQVNLKYLLKLKFNFKTSLW

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

116 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

ADP-ribosyltransferase diphtheria toxin-like 1, ADP-ribosyltransferase diphtheria toxin-like 10, ADPRT, ADPRT 1, ARTD1, ARTD10, DNA ADP-ribosyltransferase PARP1, NAD(+) ADP-ribosyltransferase 1, PARP-1, PARP-10, PARP10, Poly [ADP-ribose] polymerase 1, Poly [ADP-ribose] polymerase 10, Poly[ADP-ribose] synthase 1, PPOL, Protein mono-ADP-ribosyltransferase PARP10, Protein poly-ADP-ribosyltransferase PARP1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (7)

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Western Blot - Anti-PARP1 Antibody (A87972)

Western blot analysis of extracts of various cell lines, using Anti-PARP1 Antibody (A87972) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunohistochemistry - Anti-PARP1 Antibody (A87972)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-PARP1 Antibody (A87972) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-PARP1 Antibody (A87972)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-PARP1 Antibody (A87972) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-PARP1 Antibody (A87972)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-PARP1 Antibody (A87972) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.