Anti-PARP (cleaved Asp214) Antibody (A94925)

Name: Anti-PARP (cleaved Asp214) Antibody
Brand: Antibodies.com
SKU: A94925
Price: 190.0 USD
Availability: InStock

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$190

Rabbit polyclonal antibody to PARP (cleaved Asp214) for WB and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 6-8 business days.

Specifications

Name

Anti-PARP (cleaved Asp214) Antibody

Description

Rabbit polyclonal antibody to PARP (cleaved Asp214).

Specificity

This antibody detects endogenous levels of fragment of activated PARP resulting from cleavage adjacent to Asp214.

Applications

WB, ELISA

Dilutions

WB: 1:500-1:1000, ELISA: 1:20000

Reactivity

Human, Mouse

Immunogen

Synthetic peptide derived from human PARP (amino acids 165-214).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing peptide.

Molecular Weight

24kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ADP-ribosyltransferase diphtheria toxin-like 1, ADP-ribosyltransferase diphtheria toxin-like 10, ADPRT, ADPRT 1, ARTD1, ARTD10, DNA ADP-ribosyltransferase PARP1, NAD(+) ADP-ribosyltransferase 1, PARP-1, PARP-10, PARP10, Poly [ADP-ribose] polymerase 1, Poly [ADP-ribose] polymerase 10, Poly[ADP-ribose] synthase 1, PPOL, Protein mono-ADP-ribosyltransferase PARP10, Protein poly-ADP-ribosyltransferase PARP1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (7)

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Western Blot - Anti-PARP (cleaved Asp214) Antibody (A94925)

Western blot analysis of lysates from A549 cells, treated with etoposide 25uM 24h using Anti-PARP (cleaved Asp214) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Western Blot - Anti-PARP (cleaved Asp214) Antibody (A94925)

Western blot analysis of various cells using Anti-PARP (cleaved Asp214) Antibody.

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Western Blot - Anti-PARP (cleaved Asp214) Antibody (A94925)

Western blot analysis of A549 cells using Anti-PARP (cleaved Asp214) Antibody.

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Immunohistochemistry - Anti-PARP (cleaved Asp214) Antibody (A94925)

Immunohistochemical analysis of paraffin-embedded human uterus tissue using Anti-PARP (cleaved Asp214) Antibody at 1:200 (4°C overnight). Negative control was secondary antibody only.