Anti-Parkin Antibody (A80577)

Name: Anti-Parkin Antibody
Brand: Antibodies.com
SKU: A80577
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to Parkin for WB, IHC-P, ICC/IF and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Parkin Antibody

Description

Rabbit polyclonal antibody to Parkin.

Applications

WB, IHC-P, ICC/IF, ELISA

Positive Control

C6, 293T.

Dilutions

WB: 1:500-1:1,000, IHC-P: 1:50-1:200, ICC/IF: 1:50-1:200, ELISA: 1 µg/ml (starting concentration)

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant fusion protein corresponding to amino acids 1-387 of human Parkin.

Sequence

MIVFVRFNSSHGFPVEVDSDTSIFQLKEVVAKRQGVPADQLRVIFAGKELRNDWTVQNCDLDQQSIVHIVQRPWRKGQEMNATGGDDPRNAAGGCEREPQSLTRVDLSSSVLPGDSVGLAVILHTDSRKDSPPAGSPAGRSIYNSFYVYCKGPCQRVQPGKLRVQCSTCRQATLTLTQGPSCWDDVLIPNRMSGECQSPHCPGTSAEFFFKCGAHPTSDKETSVALHLIATNSRNITCITCTDVRSPVLVFQCNSRHVICLDCFHLYCVTRLNDRQFVHDPQLGYSLPCVAGCPNSLIKELHHFRILGEEQYNRYQQYGAEECVLQMGGVLCPRPGCGAGLLPEPDQRKVTCEGGNGLGCGFAFCRECKEAYHEGECSAVFEASGTT

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

52 kDa

Predicted MW

52 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% ProClin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

E3 ubiquitin-protein ligase parkin, PARK2, Parkin RBR E3 ubiquitin-protein ligase, Parkinson disease protein 2, Parkinson juvenile disease protein 2, PRKN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-Parkin Antibody (A80577)

Parkin expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-Parkin Antibody (A80577) at a dilution of 1:1000 and detected with chemiluminescence.

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Immunohistochemistry - Anti-Parkin Antibody (A80577)

Parkin expression in rat kidney tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-Parkin Antibody (A80577) at a dilution of 1:200.

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Immunohistochemistry - Anti-Parkin Antibody (A80577)

Parkin expression in mouse kidney tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-Parkin Antibody (A80577) at a dilution of 1:200.

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Immunofluorescence - Anti-Parkin Antibody (A80577)

Parkin expression in C6 cells analyzed by immunofluorescence. Staining was performed with Anti-Parkin Antibody (A80577) at a dilution of 1:50 followed by Cy3-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).