Western blot analysis of extracts of various cell lines, using Anti-PARK7/DJ1 Antibody (A12745) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST.
Western blot analysis of extracts from normal (control) and DJ-1/PARK7 knockout (KO) 293T cells, using Anti-PARK7/DJ1 Antibody (A12745) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.