Mouse monoclonal [C-11] antibody to pan Cytokeratin (PE).
Specificity
This antibody reacts with Cytokeratin peptides 4, 5, 6, 8, 10, 13, and 18. Cytokeratins are members of intermediate filaments subfamily intracellular proteins represented in epithelial tissues.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1 µg/ml; Intracellular staining.
Reactivity
Mammalian
Immunogen
Keratin-enriched preparation from human epidermoid carcinoma cell line A431.
Host
Mouse
Clonality
Monoclonal
Clone ID
C-11
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
CK-20, CK-2P, Cytokeratin-20, Cytokeratin-2P, K20, K2P, K76, Keratin, type I cytoskeletal 20, Keratin, type II cytoskeletal 2 oral, Keratin-20, Keratin-76, KRT20, KRT2B, KRT2P, KRT76, Protein IT, Type-II keratin Kb9
Separation of HT-29 cells stained with Anti-pan Cytokeratin Antibody [C-11] (PE) (red-filled) from HT-29 cells stained using Mouse IgG1 isotype control (PE) antibody (black-dashed) in flow cytometric analysis of HT-29 human Caucasian colon adenocarcinoma cell line.
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