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Anti-pan Cytokeratin Antibody [AE-1] - BSA and Azide free (A254152)

Mouse monoclonal [AE-1] antibody to pan Cytokeratin for Flow Cytometry, IF, WB and IHC-P.

Alternative Formats and ConjugatesAlternative Formats

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.
Name
Anti-pan Cytokeratin Antibody [AE-1] - BSA and Azide free
Description
Mouse monoclonal [AE-1] antibody to pan Cytokeratin.
Specificity
This antibody recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI , 48, 46, 45, and 40kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
Applications
Flow Cytometry, IF, WB, IHC-P
Dilutions
Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml
Reactivity
Human, Monkey, Bovine, Canine, Rabbit, Mouse, Rat, Chicken, Turtle
Immunogen
Human epidermal keratin.
Host
Mouse
Clonality
Monoclonal
Clone ID
AE-1
Isotype
IgG1
Light Chains
kappa
Conjugate

Unconjugated

Purification
Protein A/G chromatography.
Concentration
1 mg/ml
Molecular Weight
40-56.5 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
This monoclonal antibody is also available in a different formulation with BSA and Sodium Azide - Anti-pan Cytokeratin Antibody [AE-1] (A250972).
Synonyms
CK-20, CK-2P, Cytokeratin-20, Cytokeratin-2P, K20, K2P, K76, Keratin, type I cytoskeletal 20, Keratin, type II cytoskeletal 2 oral, Keratin-20, Keratin-76, KRT20, KRT2B, KRT2P, KRT76, Protein IT, Type-II keratin Kb9
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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