Mouse monoclonal [PAC-1] antibody to PAC-1 (PerCP).
Specificity
This antibody recognizes an extracellular activation-induced conformational epitope PAC-1 on CD41/CD61 complex (gpIIb/IIIa), also known as integrin alpha IIb beta 3, a receptor which mediates platelet aggregation.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Human platelets.
Host
Mouse
Clonality
Monoclonal
Clone ID
PAC-1
Isotype
IgM
Light Chains
kappa
Conjugate
PerCP
Excitation: 482nm, Emission: 675nm
Purification
This antibody is conjugated with PerCP under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 8, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
C21-LRP, C21LRP, Chromosome 21 leucine-rich protein, Down syndrome critical region protein 2, DSCR2, PAC1, Proteasome assembly chaperone 1
Flow cytometric analysis of PHA stimulated human peripheral whole blood stained with Anti-PAC-1 Antibody [PAC-1] (PerCP) (10µl reagent per 100µl of peripheral whole blood).
Separation of CD41/CD61 positive thrombocytes (red-filled) from CD41/CD61 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of PHA stimulated human peripheral whole blood using Anti-PAC-1 Antibody [PAC-1] (PerCP) (10µl reagent per 100µl of peripheral whole blood).
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