Mouse monoclonal [NGFR5] antibody to p75 NGF Receptor (APC-Cyanine 7).
Specificity
This antibody, originally known as clone C34C, recognizes an intracellular epitope, between amino acids 1 - 160, of CD271, a 75 kDa transmembrane glycoprotein of the TNFR superfamily.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests; Extracellular and Intracellular staining.
Reactivity
Human, Rabbit, Feline, Non-Human Primates, Ferret
Cross Reactivity
This antibody does not cross react with Mouse or Rat.
Immunogen
Purified CD271 protein isolated from human melanoma cell line A875.
Host
Mouse
Clonality
Monoclonal
Clone ID
NGFR5
Isotype
IgG1
Conjugate
APC-Cyanine 7
Excitation: 650nm, Emission: 774nm
Purification
This antibody is conjugated with tandem dye APC-Cyanine 7 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
75 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Separation of SK-MEL-30 cells stained with Anti-p75 NGF Receptor Antibody [NGFR5] (APC-Cyanine 7) (4 µl reagent per million cells in 100 µl of cell suspension, red-filled) from SK-MEL-30 cells stained with Mouse IgG1 [MOPC-21] (APC-Cyanine 7) - Isotype Control (concentration in sample is 5 µg/ml, same as Anti-p75 NGF Receptor Antibody [NGFR5] (APC-Cyanine 7) concentration, black-dashed) in flow cytometry analysis (intracellular staining) of SK-MEL-30 cell suspension.
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