ChIP-Seq: 4 µg/30 µg chromatin, Flow Cytometry: 1.5 µg per 1 x 106 cells in a 150 µl volume, IHC: 1:1,000-1:5,000, IP: 2-5µg/mg lysate, WB: 1:2,000-1:10,000
Reactivity
Human
Immunogen
Synthetic peptide within amino acids 50-100 of human p53.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Tris-Citrate/Phosphate Buffer, pH 7-8, with 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
General Notes
This antibody was affinity purified using the immunising peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1.0 mg of IgG.
Chromatin from MCF-7 cells was immunoprecipitated with Anti-p53 Antibody (A295374) and analyzed by DNA sequencing. The figure illustrates the peak distribution of p53 binding within a 250 Kb region of chromosome 6 as detected using Anti-p53 Antibody (A295374). ChIP-seq validation performed by Active Motif, Carlsbad, CA.
Jurkat cells were fixed in 1.5% PFA, and permeabilized in 90% Methanol. 1 X 10^6 cells were stained with 1.5 µg anti-KLH IgG control or Anti-p53 Antibody (A295374) and secondary FITC-conjugated goat anti-rabbit (in a 150µl reaction). anti-KLH control IgG (black); anti-p53 (red).
Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Anti-p53 Antibody (A295374) was used for IP at 3 µg per reaction. p53 was also immunoprecipitated using another rabbit anti-P53 antibody. For blotting immunoprecipitated p53, Anti-p53 Antibody (A295374) was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Samples: Whole cell lysate (5, 15 and 50 µg) from HEK293T cells prepared using NETN lysis buffer. Antibody: Anti-p53 Antibody (A295374) was used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
![Immunohistochemistry - Anti-p53 Antibody [TP53/2092R] (A250192) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250192_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-p53 Antibody [rBP53-12] (A250176) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250176_1.jpg?profile=product_alternative)
