Anti-p53 Antibody (A16771)

Name: Anti-p53 Antibody
Brand: Antibodies.com
SKU: A16771
Price: 345.0 USD
Availability: InStock

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$345

Rabbit polyclonal antibody to p53 for WB, IHC, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

ER Marker Mitochondrial Marker

Specifications

Name

Anti-p53 Antibody

Description

Rabbit polyclonal antibody to p53.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:1,000-1:5,000

Reactivity

Human, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-393 of human p53 (P04637).

Sequence

MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

53 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Antigen NY-CO-13, Cellular tumor antigen p53, Phosphoprotein p53, TP53, Tumor suppressor p53

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Immunohistochemistry - Anti-p53 Antibody (A16771)

Immunohistochemistry analysis of paraffin-embedded human colon tissue (negative control sample) using Anti-p53 Antibody (A16771) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-p53 Antibody (A16771)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-p53 Antibody (A16771) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Western Blot - Anti-p53 Antibody (A16771)

Immunoprecipitation analysis of 200µg extracts of 293T cells using 3µg of Anti-p53 Antibody (A16771). This Western blot was performed on the immunoprecipitate using Anti-p53 Antibody (A16771) at a dilution of 1:3000.

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