Rabbit polyclonal antibody to p44 / 42 MAP Kinase (Phospho-Tyr204)
Specificity
The antibody detects endogenous level of p44 / 42 MAP Kinase only when phosphorylated at tyrosine 204.
Applications
WB, IHC, IF
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of tyrosine 204 (T-E-Y(p)-V-A) derived from Human p44 / 42 MAP Kinase.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Predicted MW
42 44 kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Synonyms
Interferon-induced protein 44, Microtubule-associated protein 44, MTAP44
Immunofluorescence staining of methanol-fixed Hela cells showing centrosome and nuclear staining using p44/42 MAP Kinase (Phospho-Tyr204) Antibody #11246.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase (Phospho-Tyr204) Antibody #11246 (left) or the same antibody preincubated with blocking peptide #51246 (right).
Western blot analysis of extracts from SK-BR-3 cells, treated with insulin and EGF, and pre-treated with U0126 and LY294002 cells using p44/42 MAP Kinase (Phospho-Tyr204) Antibody #11246.
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