Anti-p27 KIP 1 Antibody [SPM348] - BSA and Azide free
Description
Mouse monoclonal [SPM348] antibody to p27 KIP 1.
Specificity
This antibody recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This antibody is excellent for staining of formalin-fixed tissues.
Applications
IF, IHC-P
Dilutions
IF: 1-2 µg/ml, IHC-P: 1-2 µg/ml
Reactivity
Human, Mouse, Rat, Monkey
Immunogen
Purified GST-p27 fusion protein of mouse origin.
Host
Mouse
Clonality
Monoclonal
Clone ID
SPM348
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
1 mg/ml
Molecular Weight
25-26 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Immunofluorescent analysis of PFA fixed MCF-7 cells stained with Anti-p27 KIP 1 Antibody [SPM348] followed by Goat Anti-Mouse IgG (CF® 488). Counterstain is Phalloidin-CF® 640A.
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