p27 KIP 1 expression in Human Prostate lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-p27 KIP 1 Antibody (A84181) at 0.1µg/ml and detected by chemiluminescence.
p27 KIP 1 expression in Human Prostate analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-p27 KIP 1 Antibody (A84181) at 5µg/ml and revealed with alkaline phosphatase (AP).
p27 KIP 1 expression in Human Spleen analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-p27 KIP 1 Antibody (A84181) at 5µg/ml and revealed with alkaline phosphatase (AP).
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