Anti-p27 KIP 1 Antibody (A12605)

Name: Anti-p27 KIP 1 Antibody
Brand: Antibodies.com
SKU: A12605
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to p27 KIP 1 for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-p27 KIP 1 Antibody

Description

Rabbit polyclonal antibody to p27 KIP 1.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:20-1:50

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human CDKN1B/p27KIP1 (NP_004055.1).

Sequence

MSNVRVSNGSPSLERMDARQAEHPKPSACRNLFGPVDHEELTRDLEKHCRDMEEASQRKWNFDFQNHKPLEGKYEWQEVEKGSLPEFYYRPPRPPKGACKVPAQESQDVSGSRPAAPLIGAPANSEDTHLVDPKTDPSDSQTGLAEQCAGIRKRPATDDSSTQNKRANRTEENVSDGSPNAGSVEQTPKKPGLRRRQT

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

27 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

CDKN1B, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, KIP1, p27Kip1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-p27 KIP 1 Antibody (A12605)

Western blot analysis of extracts of various cell lines, using Anti-p27 KIP 1 Antibody (A12605) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Immunofluorescence - Anti-p27 KIP 1 Antibody (A12605)

Immunofluorescence analysis of NIH/3T3 cells using Anti-p27 KIP 1 Antibody (A12605) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-p27 KIP 1 Antibody (A12605)

Immunofluorescence analysis of U-2 OS cells using Anti-p27 KIP 1 Antibody (A12605) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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